A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity

Acc Chem Res. 2004 Jan;37(1):61-9. doi: 10.1021/ar030113y.

Abstract

A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Binding, Competitive
  • DNA / chemistry*
  • DNA / metabolism
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Netropsin / chemistry
  • Netropsin / metabolism
  • Nylons / chemistry
  • Nylons / metabolism
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / metabolism
  • Spectrometry, Fluorescence / methods*
  • Thiazoles / chemistry
  • Thiazoles / metabolism

Substances

  • Fluorescent Dyes
  • Nylons
  • Oligodeoxyribonucleotides
  • Thiazoles
  • Netropsin
  • DNA