Expression, purification of, and monoclonal antibodies to sigma factors from Escherichia coli

Methods Enzymol. 2003:370:181-92. doi: 10.1016/S0076-6879(03)70016-0.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / chemistry
  • Biochemistry / methods*
  • DNA-Directed RNA Polymerases / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics*
  • Protein Folding
  • Sigma Factor / chemistry*
  • Sigma Factor / isolation & purification*
  • Time Factors

Substances

  • Antibodies, Monoclonal
  • Sigma Factor
  • DNA-Directed RNA Polymerases