Peptidomics of the locust corpora allata: identification of novel pyrokinins (-FXPRLamides)

Peptides. 2003 Oct;24(10):1493-500. doi: 10.1016/j.peptides.2003.10.006.

Abstract

The peptidomes of the corpora allata of Locusta migratoria and Schistocerca gregaria were investigated by both matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nanoscale liquid chromatography quadrupole time-of-flight tandem mass spectrometry (nanoLC-Q-TOF MSMS). The pyrokinin (-FXPRLamide) family seems to be predominant. In addition to the known pyrokinins, we de novo sequenced four pyrokinins in L. migratoria and five in S. gregaria. In addition, one pyrokinin-like peptide (-PRLamide) was identified in S. gregaria. Besides the -(FX)PRLamides, FLRFamide-1, the allatostatins (A family) and numerous as yet unidentified peptides are also present in the corpora allata.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Corpora Allata / chemistry*
  • Grasshoppers / chemistry*
  • Insect Hormones / analysis
  • Insect Hormones / chemistry
  • Insect Proteins / analysis*
  • Insect Proteins / chemistry
  • Molecular Sequence Data
  • Neuropeptides / analysis*
  • Neuropeptides / chemistry
  • Peptide Mapping
  • Proteomics
  • Reference Values
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Insect Hormones
  • Insect Proteins
  • Neuropeptides
  • pyrokinin