VARICOSE, a WD-domain protein, is required for leaf blade development

Development. 2003 Dec;130(26):6577-88. doi: 10.1242/dev.00909.

Abstract

To gain insight into the processes controlling leaf development, we characterized an Arabidopsis mutant, varicose (vcs), with leaf and shoot apical meristem defects. The vcs phenotype is temperature dependent; low temperature growth largely suppressed defects, whereas high growth temperatures resulted in severe leaf and meristem defects. VCS encodes a putative WD-domain containing protein, suggesting a function involving protein-protein interactions. Temperature shift experiments indicated that VCS is required throughout leaf development, but normal secondary vein patterning required low temperature early in leaf development. The low-temperature vcs phenotype is enhanced in axr1-3 vcs double mutants and in vcs mutants grown in the presence of polar auxin transport inhibitors, however, vcs has apparently normal auxin responses. Taken together, these observations suggest a role for VCS in leaf blade formation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis Proteins / genetics*
  • Base Sequence
  • DNA Primers
  • Exons
  • Meristem / genetics
  • Meristem / growth & development
  • Microscopy, Electron, Scanning
  • Molecular Sequence Data
  • Plant Leaves / genetics
  • Plant Leaves / growth & development*
  • Plant Leaves / ultrastructure
  • Proline
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • Arabidopsis Proteins
  • DNA Primers
  • VARICOSE protein, Arabidopsis
  • Proline