Different polymerase chain reaction-based analyses for culture-negative endocarditis caused by Streptococcus pneumoniae

Scand J Infect Dis. 2003;35(10):757-9. doi: 10.1080/00365540310015971.

Abstract

Two cases of culture-negative infective endocarditis caused by Streptococcus pneumoniae are presented. Conventional bacteriological methods were compared with 2 different polymerase chain reaction (PCR)-based analyses: PCR amplification with primers specific for S. pneumoniae, and broad-range PCR followed by sequencing. PCR-based analyses can be a valuable supplement to traditional bacteriological analyses.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Base Sequence
  • DNA, Bacterial / analysis
  • Endocarditis, Bacterial / diagnosis*
  • Endocarditis, Bacterial / microbiology
  • Female
  • Humans
  • Male
  • Polymerase Chain Reaction / methods*
  • Streptococcal Infections / diagnosis*
  • Streptococcal Infections / microbiology
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / isolation & purification*

Substances

  • DNA, Bacterial