Abstract
Calorie restriction (CR) slows aging in numerous species. In the yeast Saccharomyces cerevisiae, this effect requires Sir2, a conserved NAD+-dependent deacetylase. We report that CR reduces nuclear NAD+ levels in vivo. Moreover, the activity of Sir2 and its human homologue SIRT1 are not affected by physiological alterations in the NAD+:NADH ratio. These data implicate alternate mechanisms of Sir2 regulation by CR.
MeSH terms
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Acetaldehyde / metabolism
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Aerobiosis
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Bacterial Proteins*
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CCAAT-Binding Factor / genetics
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CCAAT-Binding Factor / metabolism
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Caloric Restriction
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Culture Media
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DNA-Binding Proteins
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Genes, Fungal
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Genes, Reporter
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Histidine / metabolism
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Histone Deacetylases / metabolism*
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Hydro-Lyases / genetics
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Hydro-Lyases / metabolism
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Longevity
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Magnetic Resonance Spectroscopy
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NAD / metabolism*
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Silent Information Regulator Proteins, Saccharomyces cerevisiae / metabolism*
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Sirtuin 1
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Sirtuin 2
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Sirtuins / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Bacterial Proteins
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CCAAT-Binding Factor
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Culture Media
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DNA-Binding Proteins
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GAL4 protein, S cerevisiae
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HAP4 protein, S cerevisiae
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NadR protein, bacteria
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Recombinant Fusion Proteins
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Silent Information Regulator Proteins, Saccharomyces cerevisiae
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Transcription Factors
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NAD
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Histidine
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SIR2 protein, S cerevisiae
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SIRT1 protein, human
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Sirtuin 1
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Sirtuin 2
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Sirtuins
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Histone Deacetylases
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Hydro-Lyases
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imidazoleglycerolphosphate dehydratase
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Acetaldehyde