Urinary eosinophilic protein X, atopy, and symptoms suggestive of allergic disease at 3 years of age

J Allergy Clin Immunol. 2003 Oct;112(4):702-8. doi: 10.1016/s0091-6749(03)01886-4.

Abstract

Background: Urinary eosinophilic protein X (U-EPX) measurement is easy to perform in children. However, its use for prediction, diagnosis, and monitoring of asthma and atopy is unclear.

Objective: We sought to investigate the relationship between U-EPX and clinical phenotypes suggestive of allergic diseases.

Methods: U-EPX measurement (RIA), respiratory questionnaires, and skin testing were completed at age 3 years in 903 children followed prospectively from birth. Specific airway resistance was measured in 503 currently asymptomatic children by using whole-body plethysmography during tidal breathing.

Results: Nonatopic children with wheezing or eczema had slightly increased U-EPX levels compared with nonatopic asymptomatic children. U-EPX levels (geometric mean EPX/creatinine ratio) were as follows: nonatopic asymptomatic children (n = 313), 61.3 microg/mmol (95% CI, 56.4-66.6 microg/mmol); nonatopic children with wheezing (n = 148), 71.2 microg/mmol (95% CI, 63.2-80.1 microg/mmol); nonatopic children with eczema (n = 90), 65.7 microg/mmol (95% CI, 56.7-76.2 microg/mmol); and nonatopic children with wheezing and eczema (n= 86), 79.7 microg/mmol (95% CI, 67.4-94.3 microg/mmol). Children who had persistent atopy early in life had significantly higher U-EPX levels at age 3 years (nonatopic at 1 and 3 years [n = 263], 63.4 microg/mmol [95% CI, 58.4-69.0 microg/mmol]; atopic at 1 but not 3 years [n = 24], 65.1 microg/mmol [95% CI, 43.8-96.7 microg/mmol]; nonatopic at 1 year and atopic at 3 years [n = 62], 90.0 microg/mmol [95% CI, 74.6-108.4 microg/mmol]; atopic at 1 and 3 years [n = 35], 111.5 microg/mmol [95% CI, 89.2-139.3 microg/mmol]; P <.002). Atopy alone and with wheezing, eczema, or both was associated with significantly increased U-EPX levels (P <.0001). Wheezing appeared to be associated with higher U-EPX levels compared with eczema in both atopic and nonatopic children. The highest U-EPX level was found in atopic children with a history of wheezing and eczema (P <.0001). There was no relationship between U-EPX level and lung function.

Conclusion: U-EPX level reflects the presence of atopy and associated symptoms and might be useful for monitoring the progression of allergic disease.

MeSH terms

  • Animals
  • Animals, Domestic
  • Child, Preschool
  • Cough / etiology
  • Dermatitis, Atopic / etiology
  • Eczema / etiology
  • Eczema / urine
  • Environmental Exposure
  • Eosinophil-Derived Neurotoxin
  • Humans
  • Hypersensitivity / complications*
  • Hypersensitivity / diagnosis
  • Hypersensitivity / epidemiology
  • Hypersensitivity / urine*
  • Lung / physiopathology
  • Medical Records
  • Multivariate Analysis
  • Prospective Studies
  • Respiratory Sounds / etiology
  • Respiratory Sounds / physiopathology
  • Ribonucleases / urine*
  • Skin Tests

Substances

  • Eosinophil-Derived Neurotoxin
  • Ribonucleases