Abstract
In the course of our search for Acyl-CoA: cholesterol acyltransferase (ACAT) inhibitors from natural sources, a new type of ACAT inhibitor was isolated from a methanol extract of Diospyros kaki. On the basis of spectral and structural evidence, the compound was identified as pheophorbide A-methyl ester. Pheophorbide A-methyl ester inhibited ACAT activity in a dose dependent manner with an IC50 value of 1.85 microg/mL.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acyl Coenzyme A / chemistry*
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Acyl Coenzyme A / isolation & purification
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Acyl Coenzyme A / pharmacokinetics
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Animals
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Chlorophyll / analogs & derivatives*
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Chlorophyll / chemistry*
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Chlorophyll / isolation & purification
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Chlorophyll / pharmacokinetics
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Chromatography, High Pressure Liquid / methods
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Diospyros / chemistry*
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Dose-Response Relationship, Drug
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Drugs, Chinese Herbal
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Gas Chromatography-Mass Spectrometry
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Korea
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Methanol
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Methyl Ethers
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Microsomes, Liver / enzymology
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Molecular Structure
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Plant Extracts / pharmacokinetics*
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Plant Extracts / pharmacology*
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Plant Leaves / chemistry*
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Rats
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Sterol O-Acyltransferase / antagonists & inhibitors*
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Sterol O-Acyltransferase / isolation & purification*
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Sterol O-Acyltransferase / pharmacokinetics
Substances
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Acyl Coenzyme A
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Drugs, Chinese Herbal
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Methyl Ethers
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Plant Extracts
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Chlorophyll
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Sterol O-Acyltransferase
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pheophorbide a
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Methanol