Real-time PCR assay targets the 23S-5S spacer for direct detection and differentiation of Legionella spp. and Legionella pneumophila

J Clin Microbiol. 2003 Oct;41(10):4815-6. doi: 10.1128/JCM.41.10.4815-4816.2003.

Abstract

A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophila within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Typing Techniques
  • DNA, Bacterial / analysis
  • DNA, Ribosomal Spacer / analysis*
  • Humans
  • Legionella / classification*
  • Legionella / genetics
  • Legionella pneumophila / classification*
  • Legionella pneumophila / genetics
  • Legionnaires' Disease / microbiology
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 23S / genetics*
  • RNA, Ribosomal, 5S / genetics*
  • Sensitivity and Specificity
  • Sputum / microbiology

Substances

  • DNA, Bacterial
  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 23S
  • RNA, Ribosomal, 5S