Abstract
We report here the regulation of the biosynthesis and the secretion of C-type natriuretic peptide (CNP) in cultured bovine chromaffin cells. The combined treatment with protein kinase A and -C activators induced a 6-fold increase of intracellular levels of CNP-(1-103). The biosynthesized CNP-(1-103) was co-released with its mature forms, typically CNP-(51-103), upon stimulation by nicotine or depolarizing agents. This confirms the neuropeptidic character of this third member of the natriuretic peptide family, which might act as a neuromodulator or neurotransmitter.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adrenal Medulla / metabolism*
-
Animals
-
Atrial Natriuretic Factor / metabolism
-
Cattle
-
Cells, Cultured
-
Chromatography, High Pressure Liquid
-
Colforsin / pharmacology
-
In Vitro Techniques
-
Natriuretic Peptide, C-Type
-
Nerve Tissue Proteins / metabolism*
-
Protein Kinase C / metabolism
-
Protein Kinases / metabolism
-
Protein Precursors / metabolism
-
Tetradecanoylphorbol Acetate / pharmacology
Substances
-
Nerve Tissue Proteins
-
Protein Precursors
-
Natriuretic Peptide, C-Type
-
Colforsin
-
Atrial Natriuretic Factor
-
Protein Kinases
-
Protein Kinase C
-
Tetradecanoylphorbol Acetate