Characterization of bovine heparin-binding neurotrophic factor (HBNF): assignment of disulfide bonds

Biochem Int. 1992 Oct;28(1):1-9.

Abstract

The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain Chemistry
  • Carrier Proteins / chemistry*
  • Carrier Proteins / genetics
  • Carrier Proteins / isolation & purification
  • Cattle
  • Chymotrypsin
  • Cysteine Endopeptidases*
  • Cytokines / chemistry*
  • Cytokines / genetics
  • Cytokines / isolation & purification
  • Disulfides / chemistry
  • Endopeptidases
  • Molecular Sequence Data
  • Molecular Structure
  • Molecular Weight
  • Peptide Mapping
  • Protein Structure, Secondary
  • Trypsin

Substances

  • Carrier Proteins
  • Cytokines
  • Disulfides
  • pleiotrophin
  • Endopeptidases
  • Chymotrypsin
  • Trypsin
  • Cysteine Endopeptidases
  • asparaginylendopeptidase