The structure of the complex between influenza virus neuraminidase and sialic acid, the viral receptor

J N Varghese; J L McKimm-Breschkin; J B Caldwell; A A Kortt; P M Colman

doi:10.1002/prot.340140302

The structure of the complex between influenza virus neuraminidase and sialic acid, the viral receptor

Proteins. 1992 Nov;14(3):327-32. doi: 10.1002/prot.340140302.

Authors

J N Varghese¹, J L McKimm-Breschkin, J B Caldwell, A A Kortt, P M Colman

Affiliation

¹ CSIRO, Division of Biomolecular Engineering, Parkville, Victoria, Australia.

PMID: 1438172
DOI: 10.1002/prot.340140302

Abstract

Crystallographic studies of neuraminidase-sialic acid complexes indicate that sialic acid is distorted on binding the enzyme. Three arginine residues on the enzyme interact with the carboxylate group of the sugar which is observed to be equatorial to the saccharide ring as a consequence of its distorted geometry. The glycosidic oxygen is positioned within hydrogen-bonding distance of Asp-151, implicating this residue in catalysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fourier Analysis
Macromolecular Substances
N-Acetylneuraminic Acid
Neuraminidase / chemistry*
Orthomyxoviridae / chemistry
Orthomyxoviridae / enzymology*
Protein Conformation
Receptors, Virus / chemistry*
Sialic Acids / chemistry*
X-Ray Diffraction

Substances

Macromolecular Substances
Receptors, Virus
Sialic Acids
2-deoxy-2,3-dehydro-N-acetylneuraminic acid
Neuraminidase
N-Acetylneuraminic Acid