Chromosomal assignment of 38 human brain expressed sequence tags (ESTs) by analyzing fluorescently labeled PCR products from hybrid cell panels

Genomics. 1992 Nov;14(3):808-10. doi: 10.1016/s0888-7543(05)80194-6.

Abstract

We have localized 38 human brain cDNA sequences to individual human chromosomes. PCR primers were designed from expressed sequence tags and tested for specific amplification from human genomic DNA. The sizes of amplification products from DNA of somatic cell hybrid mapping panels were determined electrophoretically using an automated fluorescence detection system. Chromosomal assignments were made by discordancy analysis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Brain / metabolism*
  • Chromosome Mapping
  • Chromosomes, Human*
  • DNA
  • Gene Expression
  • Humans
  • Hybrid Cells
  • Microscopy, Fluorescence
  • Molecular Sequence Data
  • Polymerase Chain Reaction

Substances

  • DNA