Abstract
A simple and rapid procedure for screening transformant yeast colonies is described. In this method, a trace amount of plasmid DNA is isolated from a small amount of yeast cell mass; then, the presence of the exogenous DNA in each yeast colony is detected by PCR amplification.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cloning, Molecular / methods
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DNA, Recombinant / analysis*
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Electrophoresis, Agar Gel
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Humans
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Lactoferrin / genetics
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Molecular Sequence Data
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Plasmids*
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Polymerase Chain Reaction
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Saccharomyces cerevisiae / genetics*
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Transformation, Genetic*
Substances
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DNA, Recombinant
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Lactoferrin