Anchored anti-HLA class I monoclonal antibody fails to induce inhibition of PHA-activated lymphocytes proliferation

Biochem Biophys Res Commun. 1992 Oct 15;188(1):278-85. doi: 10.1016/0006-291x(92)92381-7.

Abstract

It is known that anti-HLA Class I antibodies inhibit the proliferative response of PHA-activated T-lymphocytes. We found that plastic- or sepharose-linked anti-HLA Class I monoclonal antibody 01.65 does not inhibit either [3H]Thymidine incorporation or recruitment in the cell cycle, nor does it reduce the expression of c-myc mRNA and the membrane expression of Interleukin-2 Receptor and Transferrin Receptor. Furthermore, particulate Protein Kinase C is not affected by anchored anti-HLA Class I monoclonal antibody 01.65. We suggest that anti-HLA Class I monoclonal antibody may act through crosslinking or internalization of HLA Class I antigens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / pharmacology*
  • Cell Cycle
  • Cells, Cultured
  • Histocompatibility Antigens Class I / immunology*
  • Humans
  • Kinetics
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology*
  • Lymphocytes / immunology*
  • Lymphocytes / metabolism
  • Phytohemagglutinins
  • Protein Kinase C / metabolism
  • Receptors, Interleukin-2 / biosynthesis
  • Receptors, Transferrin / biosynthesis
  • Thymidine / metabolism

Substances

  • Antibodies, Monoclonal
  • Histocompatibility Antigens Class I
  • Phytohemagglutinins
  • Receptors, Interleukin-2
  • Receptors, Transferrin
  • phytohemagglutinin-P
  • Protein Kinase C
  • Thymidine