Alternative secondary structures in the phage G4 origin of the complementary DNA strand synthesis: effects of NaCl concentration on the bleomycin-DNA interaction

Biosci Biotechnol Biochem. 1992 Mar;56(3):394-8. doi: 10.1271/bbb.56.394.

Abstract

The effects of NaCl concentration on bleomycin-induced cleavages of single-strand and double-strand DNA fragments containing the phage G4 origin of complementary DNA strand synthesis were investigated. It was found that bleomycin could be used as a reagent to analyze secondary and tertiary structures and subtle changes of DNA structures. The effects of NaCl concentration on cleavages of single-stranded DNA were distinct at every target site, indicating that the diversity of topolotical properties of DNA might change the selectivity of the bleomycin-induced DNA cleavage. These results showed alternative secondary structures within and close to the G4 origin of complementary DNA strand synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / drug effects
  • Bacteriophages / genetics
  • Bacteriophages / metabolism*
  • Base Sequence
  • Bleomycin / pharmacology*
  • DNA / analysis
  • DNA / biosynthesis*
  • DNA / drug effects
  • DNA, Viral / analysis
  • DNA, Viral / chemistry
  • DNA, Viral / drug effects*
  • Molecular Sequence Data
  • Oligonucleotides / analysis
  • Sodium Chloride / pharmacology*

Substances

  • DNA, Viral
  • Oligonucleotides
  • Bleomycin
  • Sodium Chloride
  • DNA