Separation and purification of IgG1 and IgG2 from IgG-Cohn-II fraction of human plasma by pseudobioaffinity chromatography on histidyl-AH-sepharose

Bioseparation. 1992;3(1):47-53.

Abstract

L-histidine coupled to aminohexyl-sepharose (H-AH) has been used as an affinity sorbent to separate IgG from human plasma. Two subclasses IgG1 and IgG2 were specifically bound to histidyl-AH-sepharose at pH 7.4 and eluted using 0.2 M and 1M NaCl. The specificity of the two subclasses were determined by immunoelectrophoresis. Quantitative determination of IgG1, IgG2 was carried out using radial immunodiffusion technique.

MeSH terms

  • Chromatography, Affinity*
  • Electrophoresis, Agar Gel
  • Histidine / analogs & derivatives*
  • Humans
  • Immunodiffusion
  • Immunoelectrophoresis
  • Immunoglobulin G / blood
  • Immunoglobulin G / classification
  • Immunoglobulin G / isolation & purification*
  • Isoelectric Point
  • Sensitivity and Specificity
  • Sepharose / analogs & derivatives*

Substances

  • Immunoglobulin G
  • histidyl-AH-sepharose
  • Histidine
  • Sepharose