Abstract
Pokeweed antiviral protein (PAP) is a plant-derived, highly potent ribosome inactivating protein that causes inhibition of protein translation and rapid cell death. We have previously described potent cytotoxic activity of a GnRH (gonadotropin-releasing hormone) receptor-targeted conjugate protein (GnRH-PAP) and demonstrated that cytotoxicity depended on the number of GnRH receptors and the duration of exposure. Here, we demonstrate that the GnRH-PAP conjugate was cytotoxic to three different prostate cancer cell lines, supporting the feasibility of using such hormonotoxins as novel therapeutics for hormone-responsive cancers such as prostate cancer.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / administration & dosage*
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CHO Cells
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Carcinoma / metabolism
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Carcinoma / physiopathology*
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Cell Survival / drug effects
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Cricetinae
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Drug Combinations
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Drug Delivery Systems*
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Drug Screening Assays, Antitumor
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Feasibility Studies
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Gonadotropin-Releasing Hormone / administration & dosage*
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Gonadotropin-Releasing Hormone / analogs & derivatives*
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Humans
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Male
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N-Glycosyl Hydrolases*
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Plant Proteins / administration & dosage*
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Prostatic Neoplasms / metabolism
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Prostatic Neoplasms / physiopathology*
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RNA, Messenger / metabolism
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Receptors, LHRH / genetics
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Receptors, LHRH / metabolism
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Ribosome Inactivating Proteins, Type 1
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Tumor Stem Cell Assay
Substances
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Antineoplastic Agents, Phytogenic
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Drug Combinations
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Plant Proteins
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RNA, Messenger
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Receptors, LHRH
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Ribosome Inactivating Proteins, Type 1
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Gonadotropin-Releasing Hormone
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LHRH, Lys(6)-
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N-Glycosyl Hydrolases
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pokeweed antiviral protein