Monocyte chemoattractant protein-1 (MCP-1) expression in human articular cartilage. Induction by peptide regulatory factors and differential effects of dexamethasone and retinoic acid

J Clin Invest. 1992 Aug;90(2):488-96. doi: 10.1172/JCI115885.

Abstract

Monocyte influx and activation in synovial joints are important in the pathogenesis of both degenerative and inflammatory arthropathies. In this study, we demonstrate the potential of articular cartilage to directly modulate these events. IL-1-stimulated human articular chondrocytes transcribed 0.7-kb monocyte chemoattractant protein-1 (MCP-1) mRNA. In situ hybridization of cartilage organ cultures revealed MCP-1 transcripts in chondrocytes in the superficial tangential zone within 2 h of stimulation with IL-1. Chondrocytes in deeper layers responded by 4 h and reached maximum MCP-1 mRNA levels by 8-12 h. IL-1-stimulated cartilage organ and chondrocyte monolayer cultures released functional monocyte chemotactic activity. This was neutralized by a monoclonal antibody specific for MCP-1, and was associated with the synthesis and secretion of immunoreactive 13-kD and 15-kD isoforms of MCP-1. Regulators and signal transduction pathways involved with the expression of the MCP-1 gene in chondrocytes were analyzed. Steady-state mRNA levels were increased by the known chondrocyte activators IL-1, tumor necrosis factor alpha, LPS, platelet-derived growth factor, and transforming growth factor beta. In addition, leukemia inhibitory factor induced MCP-1 gene expression and protein synthesis, identifying this cytokine as a new regulator of chondrocyte function. Dexamethasone blunted the induction of MCP-1 gene expression by IL-1 and by activators of protein kinase A as well as protein kinase C signal transduction pathways. In contrast, retinoic acid strongly increased phorbol myristate acetate-induced MCP-1 expression and potentiated the effects of IL-1 and LPS. In conclusion, chondrocytes express MCP-1 in response to factors that are present in cartilage or synovium. This provides a mechanism by which cartilage can play an active role in the initiation and progression of arthritis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cartilage, Articular / physiology*
  • Chemokine CCL2
  • Chemotactic Factors / biosynthesis*
  • Chemotactic Factors / chemistry
  • Chemotactic Factors / genetics
  • Dexamethasone / pharmacology*
  • Gene Expression / drug effects
  • Growth Inhibitors / pharmacology
  • Humans
  • Interleukin-1 / pharmacology
  • Interleukin-6*
  • Leukemia Inhibitory Factor
  • Lymphokines / pharmacology
  • Molecular Sequence Data
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / chemistry
  • Organ Culture Techniques
  • RNA, Messenger / genetics
  • Transforming Growth Factor beta / pharmacology
  • Tretinoin / pharmacology*

Substances

  • Chemokine CCL2
  • Chemotactic Factors
  • Growth Inhibitors
  • Interleukin-1
  • Interleukin-6
  • LIF protein, human
  • Leukemia Inhibitory Factor
  • Lymphokines
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Tretinoin
  • Dexamethasone