Analysis of the regions responsible for IS6110 RFLP in a single Mycobacterium tuberculosis strain

M V Mendiola; C Martín; I Otal; B Gicquel

doi:10.1016/0923-2508(92)90104-v

Analysis of the regions responsible for IS6110 RFLP in a single Mycobacterium tuberculosis strain

Res Microbiol. 1992 Oct;143(8):767-72. doi: 10.1016/0923-2508(92)90104-v.

Authors

M V Mendiola¹, C Martín, I Otal, B Gicquel

Affiliation

¹ Unité Génie Microbiologique (URA 1300 du CNRS), Institut Pasteur, Paris.

PMID: 1363676
DOI: 10.1016/0923-2508(92)90104-v

Abstract

A high degree of IS6110 restriction fragment length polymorphism (RFLP) is observed amongst the different strains of the Mycobacterium tuberculosis complex. The sequences of the IS6110 flanking regions from a M. tuberculosis strain harbouring four IS6110 copies were determined. Duplication of 3-4 nucleotides was found at the extremities of the four IS6110 copies, suggesting that IS6110 RFLP is due to transposition of the IS element. One of the copies of IS6110 analysed in the study was shown to be located at the same site in the genome of M. tuberculosis as the single copy present in an M. bovis BCG strain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence / genetics
DNA Transposable Elements / genetics
Electrophoresis, Agar Gel
Genetics, Microbial
In Vitro Techniques
Molecular Sequence Data
Mycobacterium tuberculosis / genetics*
Plasmids / genetics
Polymorphism, Restriction Fragment Length*
Restriction Mapping

Substances

DNA Transposable Elements