Developmental changes of the expression of various GABAA receptor subunits (alpha 1, alpha 3, alpha 4, beta 1-3, and gamma 2) were examined in the fetal rat cerebral cortex using in situ hybridization histochemistry. The subunits showed three main patterns of development. The alpha 1 subunit showed the first pattern, in which no expression was observed during embryonic development. The alpha 4 and beta 1 subunits showed the second pattern, in which expression was observed in both the undifferentiated neuroepithelium and the developing cortical layers. The alpha 3, beta 2, beta 3, and gamma 2 subunits showed the third pattern, in which expression was only seen in the developing cortical layers. These findings strongly suggest the following: (i) the alpha 1 subunit is involved in GABAergic transmission in the mature cerebral cortex; (ii) the alpha 4 and beta 1 subunits are involved in both the differentiation of the neuroepithelium and the development of the cortical plate, and (iii) the alpha 3, beta 2, beta 3, and gamma 2 subunits are involved in the development of the cortical plate. Subunits already expressed on embryonic day 13 (beta 1, beta 3, and gamma 2) appear especially likely to have a special role in neuronal development.