[Study of the structural-functional organization of the natural variola virus genome. I. Cloning HindIII- and XhoI-fragments of viral DNA and sequencing HindIII -M, -L, -I fragments]

S N Shchelkunov; V M Blinov; A V Totmenin; S S Marennikova; A A Kolykhalov; I V Frolov; V E Chizhikov; V V Gutorov; P V Gashnikov; E F Belanov

[Study of the structural-functional organization of the natural variola virus genome. I. Cloning HindIII- and XhoI-fragments of viral DNA and sequencing HindIII -M, -L, -I fragments]

Mol Biol (Mosk). 1992 Sep-Oct;26(5):1099-115.

[Article in Russian]

Authors

S N Shchelkunov, V M Blinov, A V Totmenin, S S Marennikova, A A Kolykhalov, I V Frolov, V E Chizhikov, V V Gutorov, P V Gashnikov, E F Belanov, et al.

PMID: 1335120

Abstract

HindIII and XhoI genome fragments of variola major virus strain India-1967 were inserted into the bacterial plasmids and cosmid. Sequencing and computer analysis of the region of HindIII M, L, and I DNA fragments of the virus studied have been carried out.

Publication types

English Abstract

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Cosmids
DNA, Viral / genetics*
Deoxyribonuclease HindIII / genetics
Deoxyribonucleases, Type II Site-Specific / genetics
Genes, Viral
Molecular Sequence Data
Plasmids
Restriction Mapping
Structure-Activity Relationship
Variola virus / genetics*

Substances

DNA, Viral
Deoxyribonuclease HindIII
CTCGAG-specific type II deoxyribonucleases
Deoxyribonucleases, Type II Site-Specific