Vasoactive intestinal peptide enhances phorbol myristate acetate-induced chemiluminescence in human lymphocytes

Life Sci. 1992;51(23):1803-10. doi: 10.1016/0024-3205(92)90051-p.

Abstract

Phorbol-myristate-acetate (PMA) induced in lymphocytes the production or reactive oxygen intermediates in a process which was stimulated by the presence of vasoactive intestinal peptide (VIP) in a dose-dependent response at VIP concentrations in the range 10(-11)-10(-8) M. The dissociation constant for the high-affinity receptors of VIP agreed with the ID50 of the activation of adenylate cyclase, and the ID50 for the stimulation by VIP of PMA-induced chemiluminescence, which were close to 0.2 nM VIP. Forskolin produced in lymphocytes an effect quite similar to VIP. A comparison of the response to VIP and forskolin of lymphocytes and monocytes showed that, in contrast to forskolin, VIP failed to induce the above described effect in monocytes. A possible mechanism involving protein kinase C, which is activated by PMA, and an intracellular signal linked to VIP receptors is pointed out. This study further supports a role for VIP as a mediator in the neuroimmune system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Colforsin / pharmacology
  • Concanavalin A / pharmacology
  • Cyclic AMP / metabolism
  • Drug Synergism
  • Humans
  • Luminescent Measurements
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Protein Kinase C / physiology
  • Superoxides / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology*
  • Vasoactive Intestinal Peptide / pharmacology*

Substances

  • Concanavalin A
  • Superoxides
  • Colforsin
  • Vasoactive Intestinal Peptide
  • Cyclic AMP
  • Protein Kinase C
  • Adenylyl Cyclases
  • Tetradecanoylphorbol Acetate