Examination of the buoyant density of hepatitis C virus by the polymerase chain reaction

J Virol Methods. 1992 Sep;39(3):279-89. doi: 10.1016/0166-0934(92)90101-i.

Abstract

Sucrose and cesium chloride density gradients were used to fractionate hepatitis C virus (HCV) infectious chimpanzee plasma. The fractionated plasma was then evaluated for HCV RNA sequences using cDNA synthesis and the polymerase chain reaction (cDNA/PCR). cDNA/PCR detectable HCV RNA was identified repeatedly in two regions. One region was at the top of the gradients with a buoyant density of < or = 1.03 g/cm3, the other at a density of approximately 1.18-1.21 g/cm3.

MeSH terms

  • Animals
  • Base Sequence
  • Centrifugation, Density Gradient*
  • Cesium
  • Chlorides*
  • Hepacivirus / chemistry*
  • Hepacivirus / isolation & purification
  • Molecular Sequence Data
  • Pan troglodytes
  • Polymerase Chain Reaction*
  • RNA, Viral / blood
  • RNA, Viral / chemistry*
  • RNA, Viral / isolation & purification
  • Sucrose

Substances

  • Chlorides
  • RNA, Viral
  • Cesium
  • Sucrose
  • cesium chloride