Chronic ethanol intake inhibits both the vasoactive intestinal peptide binding and the associated cyclic AMP production in rat enterocytes

Gen Pharmacol. 1992 Jul;23(4):607-11. doi: 10.1016/0306-3623(92)90135-7.

Abstract

1. Chronic ethanol intake during 6, 8, 10 or 12 weeks resulted in a decrease of 125I-vasoactive intestinal peptide (VIP) binding to rat enterocytes. 2. Native peptide displaced 125I-VIP binding to enterocytes, exhibiting a IC50 at about 4 nM native VIP in control and ethanol-treated animals. 3. The number of binding sites in ethanol-treated animals were significantly diminished when compared to control animals. This reduction is observed in both the high-affinity and the low-affinity binding sites. 4. Increasing concentrations of native VIP produced a similar cyclic AMP rise in enterocytes from control or ethanol-treated rats during 6 weeks. However, after 8 weeks of ethanol treatment, a significant decrease in cyclic AMP production stimulated by VIP was observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cyclic AMP / biosynthesis*
  • DNA / metabolism
  • Epithelial Cells
  • Epithelium / drug effects
  • Epithelium / metabolism
  • Ethanol / blood
  • Ethanol / pharmacology*
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / metabolism
  • Iodine Radioisotopes
  • Male
  • Proteins / metabolism
  • Rats
  • Rats, Wistar
  • Vasoactive Intestinal Peptide / metabolism*

Substances

  • Iodine Radioisotopes
  • Proteins
  • Vasoactive Intestinal Peptide
  • Ethanol
  • DNA
  • Cyclic AMP