PCR cloning of the cDNA of rabbit skeletal muscle protein phosphatase inhibitor-2

Biochem Biophys Res Commun. 1992 Jul 31;186(2):1168-70. doi: 10.1016/0006-291x(92)90869-m.

Abstract

The coding region of the cDNA of protein phosphatase inhibitor-2 was determined by polymerase chain reaction amplification. The cDNA clone consisted of 621 nucleotides, and encoded 204 amino acids. The deduced amino-acid sequence was identical with that of the sequence reported by chemical sequencing methods.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular / methods
  • DNA / genetics*
  • DNA / isolation & purification
  • Molecular Sequence Data
  • Muscle Proteins / genetics*
  • Muscles / physiology*
  • Oligodeoxyribonucleotides
  • Phosphoprotein Phosphatases / antagonists & inhibitors
  • Polymerase Chain Reaction / methods
  • Proteins / genetics*
  • Rabbits

Substances

  • Muscle Proteins
  • Oligodeoxyribonucleotides
  • Proteins
  • protein phosphatase inhibitor-2
  • DNA
  • Phosphoprotein Phosphatases

Associated data

  • GENBANK/M95581