Identification of the glycoprotein H gene of murine cytomegalovirus

J Gen Virol. 1992 Jul:73 ( Pt 7):1849-54. doi: 10.1099/0022-1317-73-7-1849.

Abstract

Partial sequencing of the HindIII C fragment of murine cytomegalovirus (MCMV) revealed an open reading frame of 2172 nucleotides in length encoding a 724 amino acid protein with a predicted Mr of 80.4K. Analysis of the predicted amino acid sequence revealed homology with glycoprotein H (gH) from a number of other herpesviruses. MCMV gH showed strongest amino acid identity with human (H) CMV and human herpesvirus 6 gH, and less identity with the gH protein sequences of Epstein-Barr virus, varicella-zoster virus and herpes simplex virus type 1. The greatest identity between MCMV and HCMV gH occurs in the C-terminal region. The MCMV gH is characterized by having a 14 amino acid signal sequence, a 23 amino acid transmembrane region, a seven amino acid positively charged cytoplasmic anchor sequence and eight putative N-linked glycosylation sites. Comparison of MCMV gH with that of HCMV indicates that there are 12 conserved cysteine residues and three conserved potential N-linked glycosylation sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cytomegalovirus / genetics*
  • Genes, Viral*
  • Glycoproteins / chemistry
  • Glycoproteins / genetics*
  • Mice
  • Molecular Sequence Data
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / genetics*

Substances

  • Glycoproteins
  • Viral Envelope Proteins

Associated data

  • GENBANK/D10089
  • GENBANK/L07666
  • GENBANK/L07667
  • GENBANK/L07668
  • GENBANK/L07669
  • GENBANK/L07670
  • GENBANK/L07671
  • GENBANK/L07672
  • GENBANK/L07673
  • GENBANK/M77002