Hydrocortisone-induced increase of PDGF beta-receptor expression in a human malignant mesothelioma cell line

Exp Cell Res. 1992 May;200(1):83-8. doi: 10.1016/s0014-4827(05)80074-6.

Abstract

The effect of hydrocortisone (HC) on PDGF beta-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF beta-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF beta-receptor gene. The expression of PDGF beta-receptor protein was also elevated by HC as demonstrated with an immunoblotting assay. However, the number of PDGF-BB binding sites on the cell surface of Mero-14 remained unchanged upon HC treatment. These results suggest that steroid hormones can regulate PDGF receptor expression in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects*
  • Humans
  • Hydrocortisone / pharmacology*
  • Immunoblotting
  • Mesothelioma / metabolism
  • Protein Biosynthesis
  • RNA, Messenger / analysis
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism*
  • Receptors, Platelet-Derived Growth Factor
  • Transcription, Genetic / drug effects
  • Tumor Cells, Cultured / drug effects

Substances

  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Platelet-Derived Growth Factor
  • Hydrocortisone