Endothelin stimulates both cAMP formation and phosphatidylinositol hydrolysis in cultured embryonic bovine tracheal cells

K Oda; Y Fujitani; T Watakabe; T Inui; T Okada; Y Urade; E Okuda-Ashitaka; S Ito

doi:10.1016/0014-5793(92)80244-b

Endothelin stimulates both cAMP formation and phosphatidylinositol hydrolysis in cultured embryonic bovine tracheal cells

FEBS Lett. 1992 Mar 9;299(2):187-91. doi: 10.1016/0014-5793(92)80244-b.

Authors

K Oda¹, Y Fujitani, T Watakabe, T Inui, T Okada, Y Urade, E Okuda-Ashitaka, S Ito

Affiliation

¹ International Research Laboratories, Ciba-Geigy Ltd., Takarazuka, Japan.

PMID: 1312041
DOI: 10.1016/0014-5793(92)80244-b

Abstract

Embryonic bovine tracheal (EBTr) cells were found to possess receptors for endothelin (ET) of ET-1-selective (ETA) subtype with a Kd for ET-1 of 114 pM and a Bmax of 12.9 fmol/10(5) cells. Stimulation of EBTr cells with 100 pM to 100 nM ET-1 increased the contents of both inositol phosphates and cAMP in a concentration-dependent manner, indicating that the receptors are coupled to both phosphatidylinositol hydrolysis and cAMP formation in EBTr cells.

MeSH terms

Animals
Cattle
Cells, Cultured
Cyclic AMP / biosynthesis*
Endothelins / pharmacology*
Hydrolysis
Phosphatidylinositols / biosynthesis*
Receptors, Cell Surface / metabolism
Receptors, Endothelin
Trachea / cytology
Trachea / metabolism*

Substances

Endothelins
Phosphatidylinositols
Receptors, Cell Surface
Receptors, Endothelin
Cyclic AMP