Highly restricted localization of RNA polymerase II within a locus control region of a tissue-specific chromatin domain

Mol Cell Biol. 2003 Sep;23(18):6484-93. doi: 10.1128/MCB.23.18.6484-6493.2003.

Abstract

RNA polymerase II (Pol II) can associate with regulatory elements far from promoters. For the murine beta-globin locus, Pol II binds the beta-globin locus control region (LCR) far upstream of the beta-globin promoters, independent of recruitment to and activation of the betamajor promoter. We describe here an analysis of where Pol II resides within the LCR, how it is recruited to the LCR, and the functional consequences of recruitment. High-resolution analysis of the distribution of Pol II revealed that Pol II binding within the LCR is restricted to the hypersensitive sites. Blocking elongation eliminated the synthesis of genic and extragenic transcripts and eliminated Pol II from the betamajor open reading frame. However, the elongation blockade did not redistribute Pol II at the hypersensitive sites, suggesting that Pol II is recruited to these sites. The distribution of Pol II did not strictly correlate with the distributions of histone acetylation and methylation. As Pol II associates with histone-modifying enzymes, Pol II tracking might be critical for establishing and maintaining broad histone modification patterns. However, blocking elongation did not disrupt the histone modification pattern of the beta-globin locus, indicating that Pol II tracking is not required to maintain the pattern.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylation
  • Animals
  • Binding Sites
  • Cells, Cultured
  • Chromatin / metabolism*
  • Erythroid Precursor Cells / physiology
  • Globins / genetics
  • Globins / metabolism
  • Histone Acetyltransferases
  • Histones / metabolism
  • Locus Control Region / physiology*
  • Methylation
  • Mice
  • Organ Specificity
  • Peptide Chain Elongation, Translational
  • Promoter Regions, Genetic
  • RNA Polymerase II / genetics
  • RNA Polymerase II / metabolism*
  • TATA-Binding Protein Associated Factors / immunology
  • TATA-Binding Protein Associated Factors / metabolism
  • Transcription Factor TFIID / immunology
  • Transcription Factor TFIID / metabolism
  • Transcription, Genetic

Substances

  • Chromatin
  • Histones
  • TATA-Binding Protein Associated Factors
  • Transcription Factor TFIID
  • Globins
  • Histone Acetyltransferases
  • TATA-binding protein associated factor 250 kDa
  • RNA Polymerase II