Structure of the bacteriophage T4 DNA adenine methyltransferase

Nat Struct Biol. 2003 Oct;10(10):849-55. doi: 10.1038/nsb973. Epub 2003 Aug 24.

Abstract

DNA-adenine methylation at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as bacterial virulence. We report here the crystal structures of the bacteriophage T4Dam DNA adenine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a seven-stranded catalytic domain that harbors the binding site for AdoHcy and a DNA binding domain consisting of a five-helix bundle and a beta-hairpin that is conserved in the family of GATC-related MTase orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure provides a rare snapshot of an enzyme poised for linear diffusion along the DNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacteriophage T4 / enzymology*
  • Binding Sites
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • S-Adenosylhomocysteine / metabolism
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / chemistry*
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / metabolism

Substances

  • S-Adenosylhomocysteine
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)

Associated data

  • PDB/1Q0S
  • PDB/1Q0T