Estimation of time since death with the change in chloride concentration in postmortem blood is the topic of discussion in the study. Querido (Forensic Sci Int 45 (1990) 117) had demonstrated a highly significant double logarithmic linear relationship between the plasma chloride concentration and postmortem interval in Wistar rats. Henceforth, the present study was carried out to substantiate this propensity in humans. Chloride concentration in postmortem blood of 474 subjects revealed a highly significant relationship between logarithm of plasma chloride concentration and logarithm of the time since death during 3-58 h of death. Notwithstanding, time since death can be predicted from plasma chloride concentration with standard error of estimate of 2.1 h but factors like environmental temperature, cause of death, age and gender influences it significantly.