Cross-linking strategy for molecular recognition and fluorescent sensing of a multi-phosphorylated peptide in aqueous solution

J Am Chem Soc. 2003 Aug 27;125(34):10184-5. doi: 10.1021/ja036317r.

Abstract

In the research field of molecular recognition, selective recognition and sensing of phosphorylated protein surfaces is strongly desirable both for elucidation of protein-protein recognition at the molecular level and for regulation of signal transduction through protein surfaces. Here we describe a new strategy for molecular recognition of a multi-phosphorylated peptide using intrapeptide cross-linking on the basis of coordination chemistry. The present artificial receptor can selectively bind to doubly phosphorylated peptide through multiple-point interactions and fluorescently sense the binding event with an association constant of more than 106 M-1 in neutral aqueous solution.

MeSH terms

  • Circular Dichroism
  • Cross-Linking Reagents / chemistry*
  • Fluorescent Dyes / chemistry*
  • Kinetics
  • Organometallic Compounds / chemistry
  • Phosphopeptides / analysis*
  • Protein Structure, Secondary
  • Solutions
  • Spectrometry, Fluorescence / methods
  • Water / chemistry
  • Zinc / chemistry

Substances

  • Cross-Linking Reagents
  • Fluorescent Dyes
  • Organometallic Compounds
  • Phosphopeptides
  • Solutions
  • Water
  • Zinc