Liquid-solid transition in nuclei of protein crystals

Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10254-7. doi: 10.1073/pnas.1334069100. Epub 2003 Aug 18.

Abstract

It is generally assumed that crystallization begins with a small, crystalline nucleus. For proteins this paradigm may not be valid. Our numerical simulations show that under conditions typically used to produce protein crystals, small clusters of model proteins (particles with short-range, attractive interactions) cannot maintain a crystalline structure. Protein crystal nucleation is therefore an indirect, two-step process. A nucleus first forms and grows as a disordered, liquid-like aggregate. Once the aggregate grows beyond a critical size (about a few hundred particles) crystal nucleation becomes possible.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Computer Simulation
  • Crystallization
  • Proteins / chemistry*

Substances

  • Proteins