Fluorescence microscopic visualization of a DNA-cationic fullerene complex

Nucleic Acids Symp Ser. 2000:(44):173-4. doi: 10.1093/nass/44.1.173.

Abstract

Cationic fullerene derivative 1, which is soluble in aqueous medium, bound to double stranded DNA. Observation of Coliphage T4 DNA in the absence and presence of 1 by fluorescence microscopy suggested that 1 bound to DNA along its groove. Upon irradiation of visible light on the DNA complex of 1, DNA was cleaved dramatically. The process of DNA photo-cleavage could be monitored continuously by fluorescence microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage T4 / chemistry
  • Binding Sites
  • Cations
  • DNA / chemistry*
  • DNA / radiation effects
  • DNA / ultrastructure*
  • DNA, Viral / chemistry
  • DNA, Viral / radiation effects
  • DNA, Viral / ultrastructure
  • Fullerenes / chemistry*
  • Fullerenes / radiation effects
  • In Vitro Techniques
  • Macromolecular Substances
  • Microscopy, Fluorescence
  • Models, Molecular
  • Molecular Conformation
  • Nucleic Acid Conformation
  • Photochemistry

Substances

  • Cations
  • DNA, Viral
  • Fullerenes
  • Macromolecular Substances
  • DNA