Solid-phase synthesis of deoxyribonucleotides having a cap structure analog

M Ushioda; M Kadokura; Y Murami; T Moriguti; T Wada; K Seio; M Sekine

doi:10.1093/nass/44.1.123

Solid-phase synthesis of deoxyribonucleotides having a cap structure analog

Nucleic Acids Symp Ser. 2000:(44):123-4. doi: 10.1093/nass/44.1.123.

Authors

M Ushioda¹, M Kadokura, Y Murami, T Moriguti, T Wada, K Seio, M Sekine

Affiliation

¹ Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226, Japan.

PMID: 12903299
DOI: 10.1093/nass/44.1.123

Abstract

In this study, the solid-phase synthesis of oligodeoxyribonucleotides having a guanosine pyrophosphate cap structure (Gpp-) was achieved by using a new guanosine monophosphate unit having the DMTr group capable of estimation of coupling efficiency of the pyrophosphate bond formation. Since 7-methylguanosine base was unstable under basic conditions, Gpp-capped DNA oligomers were synthesized by using a new linker having a silanediyl bond, which allowed to release the DNA chain from the solid support by treatment with fluoride anion under neutral conditions.

MeSH terms

Methods
Molecular Structure
Oligodeoxyribonucleotides / chemical synthesis*
Oligodeoxyribonucleotides / chemistry*
RNA Cap Analogs / chemical synthesis*
RNA Cap Analogs / chemistry*

Substances

Oligodeoxyribonucleotides
RNA Cap Analogs