Abstract
A phosphorylated core-lipid A backbone oligosaccharide that carries a disaccharide remainder of the first O-antigen repeating unit was derived by strong alkaline degradation following mild hydrazinolysis of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 4 (serogroup O-1). The structure of the oligosaccharide was determined using ESI MS and NMR spectroscopy and it was demonstrated that 2-acetamido-2,6-dideoxy-D-glucose is the first monosaccharide of the O-polysaccharide that is linked to the LPS core. These data define the structure of the biological repeating unit of the O-antigen.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylglucosamine / analogs & derivatives*
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Acetylglucosamine / chemistry*
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Carbohydrate Sequence
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Fucose / analogs & derivatives*
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Fucose / chemistry*
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Lipopolysaccharides / chemistry
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Lipopolysaccharides / isolation & purification
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Molecular Sequence Data
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O Antigens / chemistry*
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Pseudomonas aeruginosa / chemistry*
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Pseudomonas aeruginosa / immunology
Substances
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Lipopolysaccharides
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O Antigens
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Fucose
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N-acetylfucosamine
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2-acetamido-2,6-dideoxyglucose
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Acetylglucosamine