Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments

Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):1013-1018. doi: 10.1099/ijs.0.02521-0.

Abstract

Two red pigment-producing bacterial strains with a metallic green sheen were isolated from a sediment sample of getbol, the Korean tidal flat. Phylogenetic analysis based on 16S rDNA sequences showed that these isolates represent a phyletic lineage within the gamma-Proteobacteria that is distantly related to the genus Hahella. No bacterial species with validly published names showed > or = 92% 16S rRNA similarity with the getbol isolates. The strains were gram-negative, chemo-organotrophic, aerobic and required NaCl (1-7%) for growth. They produced pigments with maximum absorption at 540 nm, which indicated the presence of prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The major isoprenoid quinone was ubiquinone-9. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. The DNA G + C contents ranged from 40 to 42 mol%. The combination of physiological, biochemical and chemotaxonomic data clearly separated the test strains from other phylogenetically related genera in the gamma-Proteobacteria. On the basis of polyphasic evidence from this study, it is proposed that the two getbol isolates should be classified in a novel genus, Zooshikella gen. nov., as Zooshikella ganghwensis sp. nov.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fatty Acids / metabolism
  • Gammaproteobacteria / classification*
  • Gammaproteobacteria / genetics
  • Gammaproteobacteria / isolation & purification*
  • Gammaproteobacteria / metabolism
  • Geologic Sediments / microbiology*
  • Korea
  • Microscopy, Electron, Scanning
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • Pigments, Biological / metabolism
  • Prodigiosin / metabolism
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics

Substances

  • Fatty Acids
  • Pigments, Biological
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Prodigiosin

Associated data

  • GENBANK/AY130994
  • GENBANK/AY130995