Abstract
With increased sensitivity and specificity, fluorescent assays are rapidly becoming the method of choice for nucleic acid quantification. The utility of the Typhoon scanner has now been extended to accurately measure low levels of DNA and RNA (5 ng ml(-1)) with PicoGreen and RiboGreen dyes. In addition, with a few simple modifications, autoradiographic film images can be scanned and quantified with the Typhoon series of scanners.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Autoradiography / instrumentation*
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Autoradiography / methods
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DNA / analysis*
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Equipment Failure Analysis
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Fluorescent Dyes
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Image Enhancement / instrumentation*
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Image Enhancement / methods
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In Situ Hybridization / instrumentation
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In Situ Hybridization / methods
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RNA / analysis*
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Reproducibility of Results
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Sensitivity and Specificity