[Determination of free thiols in the chimeric protein PfCP-2.9 of Plasmodium falciparum]

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2003;21(2):99-101.
[Article in Chinese]

Abstract

Objective: To determine the free thiols in the chimeric protein PfCP-2.9 of Plasmodium falciparum expressed by Pichia pastoris.

Methods: Two experiments of reverse phase HPLC and Ellman's reaction were applied to the PfCP-2.9 for the determination of its free thiols. For RP-HPLC analysis, three kinds of samples were tested: PfCP-2.9, dithiothreitol-reduced PfCP-2.9 and indoacetic acid-alkylated PfCP-2.9.

Results: Both experiments showed that there were no any free thiols present in the PfCP-2.9.

Conclusion: The disulfide bonds between cysteine residues of PfCP-2.9 were formed completely.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Protozoan / analysis*
  • Chromatography, High Pressure Liquid
  • Membrane Proteins / analysis*
  • Merozoite Surface Protein 1 / analysis*
  • Protozoan Proteins / analysis*
  • Recombinant Fusion Proteins / analysis*
  • Spectrophotometry
  • Sulfhydryl Compounds / analysis*
  • Sulfhydryl Compounds / chemistry

Substances

  • Antigens, Protozoan
  • Membrane Proteins
  • Merozoite Surface Protein 1
  • Protozoan Proteins
  • Recombinant Fusion Proteins
  • Sulfhydryl Compounds
  • apical membrane antigen I, Plasmodium