Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC

Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1466-8. doi: 10.1107/s0907444903010990. Epub 2003 Jul 23.

Abstract

The C-terminal truncated form of the S-layer protein SbsC from Geobacillus stearothermophilus, rSbsC(31-844), has been crystallized by the vapour-diffusion method using polyethylene glycol 6000 as a precipitating agent. The crystals diffract to 3 A resolution using synchrotron radiation and belong to space group P2(1), with unit-cell parameters a = 57.24, b = 98.91, c = 108.62 A, beta = 94.34 degrees. One molecule is present in the asymmetric unit, which corresponds to a solvent content of 65%. Native and heavy-atom derivative data have been collected. The Pt derivative yielded two high-occupancy sites per molecule.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Binding Sites
  • Crystallization
  • Crystallography, X-Ray
  • Geobacillus stearothermophilus / metabolism*
  • Membrane Glycoproteins / chemistry*
  • Methanosarcina / metabolism
  • Platinum / chemistry
  • Polyethylene Glycols / chemistry
  • Protein Conformation
  • Protein Structure, Tertiary
  • Temperature
  • X-Ray Diffraction

Substances

  • Bacterial Proteins
  • Membrane Glycoproteins
  • S-layer proteins
  • Polyethylene Glycols
  • Platinum