Oxime bond formation for the covalent attachment of oligonucleotides on glass support

Bioorg Med Chem Lett. 2003 Aug 18;13(16):2683-6. doi: 10.1016/s0960-894x(03)00537-7.

Abstract

The chemical attachment of oligonucleotides on glass slides has been achieved using oxime bond formation. This method has been shown very efficient by comparison with the attachment of amino-oligonucleotides via reductive amination.

MeSH terms

  • Amination
  • Glass / chemistry*
  • Image Processing, Computer-Assisted
  • In Situ Hybridization, Fluorescence
  • Models, Chemical
  • Oligonucleotides / chemical synthesis*
  • Oxidation-Reduction
  • Oximes / chemical synthesis*
  • Silanes / chemistry

Substances

  • Oligonucleotides
  • Oximes
  • Silanes