Comparison of genotyping Helicobacter pylori directly from biopsy specimens and genotyping from bacterial cultures

J Clin Microbiol. 2003 Jul;41(7):3336-8. doi: 10.1128/JCM.41.7.3336-3338.2003.

Abstract

PCR for vacA and cagA genotypes of Helicobacter pylori using DNA isolated from infected gastric biopsy specimens was approximately equal to genotyping using bacterial DNA from cultures. Inconsistent results were associated with low H. pylori density in biopsies. A higher proportion of mixed infection was found when biopsies were used.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Antigens, Bacterial / genetics
  • Bacterial Proteins / genetics
  • Bacterial Typing Techniques*
  • Biopsy*
  • Culture Media
  • DNA, Bacterial / analysis*
  • DNA, Bacterial / isolation & purification
  • Genotype
  • Helicobacter Infections / microbiology
  • Helicobacter pylori / classification*
  • Helicobacter pylori / genetics
  • Helicobacter pylori / growth & development*
  • Humans
  • Polymerase Chain Reaction / methods
  • Stomach / microbiology*

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Culture Media
  • DNA, Bacterial
  • VacA protein, Helicobacter pylori
  • cagA protein, Helicobacter pylori