Evidence for a Schiff base formation of peptides derived from RecA with single-stranded oligonucleotides containing 5-formyl-2'-deoxyuridine

T Sugiyama; A Kittaka; H Takayama; R Kuroda

doi:10.1093/nass/1.1.175

Evidence for a Schiff base formation of peptides derived from RecA with single-stranded oligonucleotides containing 5-formyl-2'-deoxyuridine

Nucleic Acids Res Suppl. 2001:(1):175-6. doi: 10.1093/nass/1.1.175.

Authors

T Sugiyama¹, A Kittaka, H Takayama, R Kuroda

Affiliation

¹ Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.

PMID: 12836321
DOI: 10.1093/nass/1.1.175

Abstract

We report some evidence for a Schiff base formation of 5-formyl-2'-deoxyuridine containing oligonucleotides with oligopeptide derived from the DNA binding site of RecA protein. We have demonstrated that cross-linked products were successfully detected by lowering the peptide concentrations. This is probably due to the reduced formation of large aggregates.

MeSH terms

Amino Acid Sequence
Base Sequence
Binding Sites
Deoxyuridine / analogs & derivatives*
Deoxyuridine / chemistry*
Molecular Sequence Data
Oligonucleotides / chemistry*
Oligonucleotides / metabolism
Peptides / chemistry*
Peptides / metabolism
Protein Structure, Secondary
Rec A Recombinases / chemistry*
Rec A Recombinases / metabolism
Schiff Bases / chemistry

Substances

FECO peptide
Oligonucleotides
Peptides
Schiff Bases
5-formyl-2'-deoxyuridine
Rec A Recombinases
Deoxyuridine