Trinuclear Cu(II) complex for internucleoside linkage-specific cleavage of RNA

Nucleic Acids Res Suppl. 2001:(1):85-6. doi: 10.1093/nass/1.1.85.

Abstract

Trinuclear Cu(II) complex of N,N,N',N',N",N"-hexa[(2-pyridyl)methyl]-1,3,5-tris(aminomethyl)benzene (L3A) exhibits remarkable substrate-specificity for hydrolysis of 2'-->5' and 3'-->5' ribonucleotides. Up(2'-->5')U is hydrolyzed much efficiently than is Up(3'-->5')U, but Ap(3'-->5')A is overwhelmingly preferable to Ap(2'-->5')A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Copper / chemistry*
  • Kinetics
  • Macromolecular Substances
  • Organometallic Compounds / chemistry*
  • Organometallic Compounds / metabolism*
  • Pyridines / chemistry*
  • Pyridines / metabolism*
  • RNA / metabolism*
  • Ribonucleases / chemistry*
  • Ribonucleases / metabolism*
  • Ribonucleosides / metabolism
  • Ribonucleotides / metabolism
  • Substrate Specificity

Substances

  • (N,N,N',N',N'',N''-hexa((2-pyridyl)methyl)-1,3,5-tris(aminomethyl)benzene)copper(II)
  • Macromolecular Substances
  • Organometallic Compounds
  • Pyridines
  • Ribonucleosides
  • Ribonucleotides
  • RNA
  • Copper
  • Ribonucleases