Evaluation of VEGF and VEGF-C expression in gastric cancer cells producing alpha-fetoprotein

J Gastroenterol. 2003;38(6):540-7. doi: 10.1007/s00535-002-1099-y.

Abstract

Background: Gastric cancers producing alpha-fetoprotein (AFP) are known to have a poor prognosis and to show a high incidence of liver metastasis. Vascular endothelial growth factor (VEGF) and its isoform VEGF-C are reported to be associated with tumor progression through an angiogenic or lymphangiogenic function. In the present study, to clarify the cellular characteristics of AFP-producing gastric cancers, the expression of VEGF and that of VEGF-C in AFP-producing gastric cancer was compared with their expression in gastric cancers that do not produce AFP.

Methods: Twenty-six patients with AFP-producing gastric cancers [AFP(+)] and 26 patients with stage-matched gastric cancers that did not produce AFP [AFP(-)] were evaluated for VEGF and VEGF-C expression and vessel density, using immunohistochemical analysis.

Results: The survival rate of the AFP(+) group was significantly worse than that of the stage-matched AFP(-) group (P < 0.05). The frequency of VEGF-C expression was significantly higher in the AFP(+) group than in the AFP(-) group (P < 0.01). There was no significant difference in VEGF expression between the AFP(+) and AFP(+) groups. The microvessel density was higher in the AFP(+) group than in the AFP(-) group (P < 0.05).

Conclusions: A higher expression of VEGF-C might be one explanation for the poorer prognosis of AFP-producing gastric cancers.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Carcinoma / metabolism*
  • Carcinoma / mortality
  • Case-Control Studies
  • Endothelial Growth Factors / metabolism*
  • Female
  • Humans
  • Immunohistochemistry
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Liver Neoplasms / secondary
  • Lymphatic Metastasis
  • Lymphokines / metabolism*
  • Male
  • Middle Aged
  • Prognosis
  • Protein Isoforms
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / mortality
  • Tumor Cells, Cultured
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factor C
  • Vascular Endothelial Growth Factors
  • alpha-Fetoproteins / biosynthesis*

Substances

  • Endothelial Growth Factors
  • Intercellular Signaling Peptides and Proteins
  • Lymphokines
  • Protein Isoforms
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factor C
  • Vascular Endothelial Growth Factors
  • alpha-Fetoproteins