Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia

K K Kim; K Y Hwang; H S Jeon; S Kim; R M Sweet; C H Yang; S W Suh

doi:10.1016/0022-2836(92)90537-t

Crystallization and preliminary X-ray crystallographic analysis of lipase from Pseudomonas cepacia

J Mol Biol. 1992 Oct 20;227(4):1258-62. doi: 10.1016/0022-2836(92)90537-t.

Authors

K K Kim¹, K Y Hwang, H S Jeon, S Kim, R M Sweet, C H Yang, S W Suh

Affiliation

¹ Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.

PMID: 1279182
DOI: 10.1016/0022-2836(92)90537-t

Abstract

Large crystals of lipase from Pseudomonas cepacia have been grown at room temperature from solutions containing 2-methyl-2,4-pentanediol and sodium citrate. They grow within two weeks to typical dimensions of 1.0 mm x 0.5 mm x 0.3 mm. The crystals belong to the monoclinic space group P2(1), with unit cell parameters a = 84.91 A, b = 47.33 A, c = 86.00 A, and beta = 116.09 degrees. And they diffract to about 1.6 A upon exposure to synchroton X-rays. X-ray data have been collected to 2.2 A Bragg spacing from a native crystal.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Burkholderia cepacia / enzymology*
Crystallization
Lipase / chemistry*
X-Ray Diffraction

Substances

Lipase