[Determination of piceid in commercial Rhizoma Polygoni Cuspidati by HPLC]

Zhongguo Zhong Yao Za Zhi. 2002 May;27(5):344-7.
[Article in Chinese]

Abstract

Objective: To improve a RP-HPLC method of determining piceid in commercial Rhizoma Polygoni Cuspidati.

Method: HPLC method was used, with C18 column (250 mm x 4.6 mm), a mixture of CH3CN-H2O (20:80) as the mobile phase, and detecting wavelength 303 nm.

Result: The linear range was 0.0066-0.0792 microgram.microL-1, r = 0.9999 (n = 5). The average recovery rate was 98.5% and RSD was 2.5% (n = 9).

Conclusion: The method is simple, accurate and reproducible. The contents of piceid in commercial Rhizoma Polygoni Cuspidati from different areas are variable and the average content is 2.23%.

Publication types

  • Comparative Study
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Drugs, Chinese Herbal / analysis*
  • Glucosides / analysis*
  • Plant Roots / chemistry
  • Plants, Medicinal / chemistry*
  • Polygonum / chemistry*
  • Quality Control
  • Rhizome / chemistry
  • Species Specificity
  • Stilbenes / analysis*

Substances

  • Drugs, Chinese Herbal
  • Glucosides
  • Stilbenes
  • polydatin