15-deoxy-delta(12,14)-prostaglandin J2 inhibits IFN-gamma-induced galectin-9 expression in cultured human umbilical vein endothelial cells

Int Arch Allergy Immunol. 2003 May;131(1):57-61. doi: 10.1159/000070436.

Abstract

Background: Galectin-9 is involved in chemotaxis and adhesion of eosinophils, and is induced in vascular endothelial cells by interferon-gamma (IFN-gamma). 15-deoxy-delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) is a ligand for peroxisome proliferator-activated receptor-gamma (PPAR-gamma), and known to modulate the expression of various genes.

Methods: We have studied the effect of 15d-PGJ(2) on the IFN-gamma-induced galectin-9 expression in human umbilical vein endothelial cells (HUVEC) in culture.

Results: 15d-PGJ(2) inhibited the IFN-gamma-induced galectin-9 expression in a PPAR-gamma-independent manner, and also inhibited the adhesion of EoL-1 cells to an HUVEC monolayer treated with IFN-gamma. 15d-PGJ(2) partially inhibited IFN-gamma-induced phosphorylation of STAT-1 in HUVEC.

Conclusions: 15d-PGJ(2) may regulate inflammatory reactions through the inhibition of galectin-9 expression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cell Adhesion / drug effects
  • Cells, Cultured / drug effects
  • DNA-Binding Proteins / drug effects
  • DNA-Binding Proteins / metabolism
  • Dose-Response Relationship, Drug
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Galectins / biosynthesis*
  • Galectins / drug effects*
  • Humans
  • Immunologic Factors / administration & dosage
  • Immunologic Factors / pharmacology*
  • Interferon-gamma / administration & dosage
  • Interferon-gamma / pharmacology*
  • Phosphorylation / drug effects
  • Polymerase Chain Reaction
  • Prostaglandin D2 / administration & dosage
  • Prostaglandin D2 / analogs & derivatives
  • Prostaglandin D2 / pharmacology*
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Receptors, Cytoplasmic and Nuclear / administration & dosage
  • Receptors, Cytoplasmic and Nuclear / antagonists & inhibitors
  • STAT1 Transcription Factor
  • Trans-Activators / drug effects
  • Trans-Activators / metabolism
  • Transcription Factors / administration & dosage
  • Transcription Factors / antagonists & inhibitors
  • Transcription, Genetic / drug effects
  • Umbilical Veins / cytology*
  • Umbilical Veins / drug effects
  • Umbilical Veins / metabolism*

Substances

  • 15-deoxy-delta(12,14)-prostaglandin J2
  • DNA-Binding Proteins
  • Galectins
  • Immunologic Factors
  • LGALS9 protein, human
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • STAT1 Transcription Factor
  • STAT1 protein, human
  • Trans-Activators
  • Transcription Factors
  • Interferon-gamma
  • Prostaglandin D2