Abstract
Mycothiol is the major thiol present in most actinomycetes and is produced from the pseudodisaccharide 1D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside (GlcNAc-Ins). A transposon mutant of Mycobacterium smegmatis shown to be GlcNAc-Ins and mycothiol deficient was sequenced to identify a putative glycosyltransferase gene designated mshA. The ortholog in Mycobacterium tuberculosis, Rv0486, was used to complement the mutant phenotype.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism
-
Cysteine
-
DNA Transposable Elements
-
Disaccharides / biosynthesis*
-
Glycopeptides
-
Glycosyltransferases / genetics*
-
Glycosyltransferases / metabolism*
-
Inositol
-
Molecular Sequence Data
-
Mutation
-
Mycobacterium smegmatis / enzymology*
-
Mycobacterium smegmatis / genetics
-
Mycobacterium tuberculosis / enzymology*
-
Mycobacterium tuberculosis / genetics
-
Pyrazoles
-
Sequence Alignment
-
Sulfhydryl Compounds
Substances
-
Bacterial Proteins
-
DNA Transposable Elements
-
Disaccharides
-
Glycopeptides
-
Pyrazoles
-
Sulfhydryl Compounds
-
mycothiol
-
Inositol
-
Glycosyltransferases
-
Cysteine